Longevity experiments with Caenorhabditis elegans worms under different temperature conditions Pamela L. Larsen (University of Southern California, Andrus Gerontology Center, Los Angeles, CA 90089-0191, USA)
TS#1 In order to raise animals to adulthood at 25.5C, the double mutant daf-2(m41); daf-12(m20) had to be used. The treatments here are shifts between the permissive (15C where the mutant is phenotypically similar to wild type) and restrictive (25.5C where the mutant is phenotypically different from wild type) temperatures. The temperature shifts occurred at specific stages/phases in the life course.
TS#2 Upshift and downshift repeat like TS#1.
Pulsbab Here the pulses were at 25.5C for 2 or 4 days and the rest of life was at 15C.
Downpul The animals were given a temperature pulse for 8 days at different advancing adult ages. The pulse was to 15C and the rest of life was spent at 25.5C.
Longpul in this experiment there were 8 groups. The animals were given a temperature pulse for 8 days at different advancing adult ages. The pulse was to 25.5C and the rest of life was spent at 15C.
Latepul Tests were with up pulses late in life. Pulse to 25.5C and the rest of life was at 15C.
